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antibody from Invitrogen Antibodies
Targeting: MAPK12
ERK6, p38gamma, PRKM12, SAPK-3, SAPK3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-105907 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-p38 MAPK gamma/delta (Tyr185, Tyr182) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references MKK6 deficiency promotes cardiac dysfunction through MKK3-p38γ/δ-mTOR hyperactivation.
Romero-Becerra R, Mora A, Manieri E, Nikolic I, Santamans AM, Montalvo-Romeral V, Cruz FM, Rodríguez E, León M, Leiva-Vega L, Sanz L, Bondía V, Filgueiras-Rama D, Jiménez-Borreguero LJ, Jalife J, Gonzalez-Teran B, Sabio G
eLife 2022 Aug 16;11
eLife 2022 Aug 16;11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-p38 MAPK gamma/delta (Tyr185, Tyr182) in EGF treated HeLa cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-p38 MAPK gamma/delta (Tyr185, Tyr182) polyclonal antibody (Product # PA5-105907).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-p38 MAPK gamma/delta (Tyr185, Tyr182) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-p38 MAPK gamma/delta (Tyr185, Tyr182) polyclonal antibody (Product # PA5-105907) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-p38 MAPK gamma/delta (Tyr185, Tyr182) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-p38 MAPK gamma/delta (Tyr185, Tyr182) polyclonal antibody (Product # PA5-105907) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-p38 MAPK gamma/delta (Tyr185, Tyr182) in rat kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-p38 MAPK gamma/delta (Tyr185, Tyr182) polyclonal antibody (Product # PA5-105907) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Author response image 2. MKK3 deletion attenuates p38g and mTOR activation and cardiac hypertrophy in MKK6-deficient hearts. (A) Heart weight to tibia length ratio in 9-week-old MCK-Cre ( n =8), Mkk6 MCK-KO (n=10) and Mkk3/6 MCK-KO ( n =10). 1-way ANOVA followed by Tukey's multiple comparison test. * P
