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Learn20184-1-AP
antibody from Invitrogen Antibodies
Targeting: MAPK12
ERK6, p38gamma, PRKM12, SAPK-3, SAPK3
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- 20184-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAPK12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAEMITGKT LFKGSDHLDQ LKEIMKVTGT PPAEFVQRLQ SDEAKNYMKG LPELEKKDFA SILTNASPLA VNLLEKMLVL DAEQRVTAGE ALAHPYFESL HDTEDEPQVQ KYDDSFDDVD RTLDEWKRVT YKEVL (216-349 aa encoded by BC015741)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.42 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 20184-1-AP (MAPK12 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 20184-1-AP (MAPK12 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using (Product # 20184-1-AP) (MAPK12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-MAPK12 (IP:20184-1-AP, 4ug; Detection:20184-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.
