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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX110241 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110241, RRID:AB_2037393
- Product name
- MCM3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Histone demethylase Kdm4b functions as a co-factor of C/EBPβ to promote mitotic clonal expansion during differentiation of 3T3-L1 preadipocytes.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Histone demethylase Kdm4b functions as a co-factor of C/EBPβ to promote mitotic clonal expansion during differentiation of 3T3-L1 preadipocytes.
Guo L, Li X, Huang JX, Huang HY, Zhang YY, Qian SW, Zhu H, Zhang YD, Liu Y, Liu Y, Wang KK, Tang QQ
Cell death and differentiation 2012 Dec;19(12):1917-27
Cell death and differentiation 2012 Dec;19(12):1917-27
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 7.5% SDS PAGE GTX110241 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM3 antibody detects MCM3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MCM3 protein stained by MCM3 antibody (GTX110241) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM3 antibody immunoprecipitates MCM3 protein in IP experiments.IP samples: HepG2 whole cell extractA. 40 £gg HepG2 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of MCM3 protein by 4 £gg MCM3 antibody (GTX110241)7.5 % SDS-PAGEThe immunoprecipitated MCM3 protein was detected by MCM3 antibody (GTX110241) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM3 antibody detects MCM3 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MCM3 antibody (GTX110241) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
