Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-34701 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCM3 Recombinant Rabbit Monoclonal Antibody (JB99-35)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Mouse thymus tissue lysate, NIH-3T3, Hela, 293T, HepG2, SH-SY-5Y, rat testis tissue, human tonsil tissue, human placenta tissue, mouse small intestine tissue, K562.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB99-35
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCM3 in mouse thymus tissue (1), NIH-3T3 cell (2) and HeLa (3)cell lysates. Samples were incubated with MCM3 monoclonal antibody (Product # MA5-34701), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCM3 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCM3 monoclonal antibody (Product # MA5-34701), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCM3 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCM3 monoclonal antibody (Product # MA5-34701), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCM3 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCM3 monoclonal antibody (Product # MA5-34701), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCM3 in paraffin-embedded mouse small intestine tissue. Samples were incubated with MCM3 monoclonal antibody (Product # MA5-34701), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCM3 in paraffin-embedded rat testis tissue. Samples were incubated with MCM3 monoclonal antibody (Product # MA5-34701), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCM3 in paraffin-embedded mouse small intestine tissue. Samples were incubated with MCM3 monoclonal antibody (Product # MA5-34701), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCM3 in paraffin-embedded human tonsil tissue. Samples were incubated with MCM3 monoclonal antibody (Product # MA5-34701), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCM3 in paraffin-embedded human placenta tissue. Samples were incubated with MCM3 monoclonal antibody (Product # MA5-34701), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MCM3 in K562 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with MCM3 monoclonal antibody (Product # MA5-34701) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
