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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-289 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-289, RRID:AB_10001243
- Product name
- Rabbit Polyclonal MCM3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references BID binds to replication protein A and stimulates ATR function following replicative stress.
MCM proteins are negative regulators of hypoxia-inducible factor 1.
Cell cycle-dependent chromatin loading of the Fanconi anemia core complex by FANCM/FAAP24.
Liu Y, Vaithiyalingam S, Shi Q, Chazin WJ, Zinkel SS
Molecular and cellular biology 2011 Nov;31(21):4298-309
Molecular and cellular biology 2011 Nov;31(21):4298-309
MCM proteins are negative regulators of hypoxia-inducible factor 1.
Hubbi ME, Luo W, Baek JH, Semenza GL
Molecular cell 2011 Jun 10;42(5):700-12
Molecular cell 2011 Jun 10;42(5):700-12
Cell cycle-dependent chromatin loading of the Fanconi anemia core complex by FANCM/FAAP24.
Kim JM, Kee Y, Gurtan A, D'Andrea AD
Blood 2008 May 15;111(10):5215-22
Blood 2008 May 15;111(10):5215-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM3 Antibody [NB100-289] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MCM3 Antibody [NB100-289] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
