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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX101338 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101338, RRID:AB_10617798
- Product name
- BCL6 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references BCL6 attenuates renal inflammation via negative regulation of NLRP3 transcription.
Chen D, Xiong XQ, Zang YH, Tong Y, Zhou B, Chen Q, Li YH, Gao XY, Kang YM, Zhu GQ
Cell death & disease 2017 Oct 26;8(10):e3156
Cell death & disease 2017 Oct 26;8(10):e3156
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Supportive validation
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: Jurkat B: Raji C: NCI-H929 7.5% SDS PAGE GTX101338 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCL6 antibody [N2C1], Internal detects BCL6 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract C. 30 ?g C8D30 whole cell lysate/extract D. 30 ?g NIH-3T3 whole cell lysate/extract E. 30 ?g Raw264.7 whole cell lysate/extract F. 30 ?g C2C12 whole cell lysate/extract7.5% SDS-PAGEBCL6 antibody [N2C1], Internal (GTX101338) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat B: K562 C: THP-1 7.5% SDS PAGE GTX101338 diluted at 1:1000
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 £gg whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 £gg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 £gg of BCL6 antibody (GTX101338) 7.5% SDS-PAGE The immunoprecipitated BCL6 protein was detected by BCL6 antibody (GTX101338) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human tonsil, using BCL6(GTX101338) antibody(10 £gg/ml).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCL6 antibody [N2C1], Internal detects BCL6 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: BCL6 stained by BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with Raji chromatin extract and 5 £gg of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.
