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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Protein array [1]
- Other assay [1]
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Validation data
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- Product number
- V3496 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Bcl6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- BCL6/1527
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the Bcl6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with Bcl6 antibody (clone BCL6/1527). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lymphoma with Bcl6 antibody (clone BCL6/1527). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Protein array validation of the Bcl6 antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Bcl6 antibody (clone BCL6/1527). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Bcl6 Antibody (clone BCL6/1527). Confirmation of Integrity and Purity of the Antibody.
