21187-1-AP

antibody from Invitrogen Antibodies

Targeting: BCL6 BCL5, BCL6A, LAZ3, ZBTB27, ZNF51

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21187-1-AP

Provider

Invitrogen Antibodies

Product name

BCL6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SLYSGLSTP PASYSMYSHL PVSSLLFSDE EFRDVRMPVA NPFPKERALP CDSARPVPGE YSRPTLEVSP NVCHSNIYSP KETIPEEARS DMHYSVAEGL KPAAPSARNA PYFPCDKASK EEERPSSEDE IALHFEPPNA PLNRKGLVSP QSPQKSDCQP NSPTESCSSK NACILQASGS PPAKSPTDPK ACNWKKYKFI VLNSLNQNAK PEGPEQAELG RLSPRAYTAP PACQPPMEPE NLDLQSPTKL SASGEDSTIP QASRLNNIVN RSMTGSPRSS SESHSPLYMH PPKCTSCGSQ SPQHAEMCLH TAGPTFPEEM GETQSEYSDS SCENGAFFCN ECDCRFSEEA S (183-532 aa encoded by BC150184)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP ( BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH 6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH 6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 21187-1-AP ( BCL6 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 21187-1-AP ( BCL6 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH6).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Ramos cells were intracellularly stained with 0.20 µg/test Anti-Human BCL6 (Product # 21187-1-AP) (red) or 0.20 µg control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1,000. Fixed with90% MeOH.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-BCL6 (IP:21187-1-AP, 4ug; Detection:21187-1-AP 1:1000) with Raji cells lysate 1400ug.