Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 21187-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCL6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP ( BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH 6).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH 6).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 21187-1-AP ( BCL6 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH6).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 21187-1-AP ( BCL6 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH6).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 Ramos cells were intracellularly stained with 0.20 µg/test Anti-Human BCL6 (Product # 21187-1-AP) (red) or 0.20 µg control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1,000. Fixed with90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-BCL6 (IP:21187-1-AP, 4ug; Detection:21187-1-AP 1:1000) with Raji cells lysate 1400ug.