24164-1-AP

antibody from Invitrogen Antibodies

Targeting: MAPK10 JNK3, p493F12, p54bSAPK, PRKM10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

24164-1-AP

Provider

Invitrogen Antibodies

Product name

JNK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSRSKRDNN FYSVEIGDST FTVLKRYQNL KPIGSGAQGI VCAAYDAILE RNVAIKKLSR PFQNQTHAKR AYRELVLMKC VNHKNIIGLL NVFTPQKSLE EFQDVYIVME LMDANLCQVI QMELDHERMS YLLYQMLCGI KHLHSAGIIH RDLKPSNIVV KSDCTLKILD FGLARTAGTS FMMTPYVVTR YYRAPEVILG MGYKENVDIW SVGCIMGEMI KGGVLFPGTD HIDQWNKVIE QLGTPCPEFM KKLQPTVRTY VENRPKYAGY SFEKLFPDVL FPADSEHNKL KASQARDLLS KMLVIDASKR ISVDEALQHP YINVWYDPSE AEAPPPKIPD KQLDEREHTI EEWKELIYKE VMDLEERTKN GVIRGQPSPL AQVQQ (1-427 aa encoded by BC130572)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 24164-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (Product # 24164-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 24164-1-AP ( JNK Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 24164-1-AP ( JNK Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-JNK (IP:24164-1-AP, 4ug; Detection:24164-1-AP 1:1000) with HeLa cells lysate 1600ug.