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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00001820-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001820-M01, RRID:AB_538368
- Product name
- Mouse Monoclonal DRIL1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ARID3A - AT rich interactive domain 3A (BRIGHT- like)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Expression profiling of more than 3500 proteins of MSS-type colorectal cancer by stable isotope labeling and mass spectrometry.
Kang UB, Yeom J, Kim HJ, Kim H, Lee C
Journal of proteomics 2012 Jun 6;75(10):3050-62
Journal of proteomics 2012 Jun 6;75(10):3050-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DRIL1 Antibody (1A11) [H00001820-M01] - analysis of ARID3A expression in transfected 293T cell line by ARID3A monoclonal antibody (M01), clone 1A11. Lane 1: ARID3A transfected lysate (63 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DRIL1 Antibody (1A11) [H00001820-M01] - Analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi ( Cat # H00001820-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody (M01), clone 1A11 (Cat # H00001820-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DRIL1 Antibody (1A11) [H00001820-M01] - ARID3A monoclonal antibody (M01), clone 1A11 Analysis of ARID3A expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DRIL1 Antibody (1A11) [H00001820-M01] - Detection limit for recombinant GST tagged ARID3A is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: DRIL1 Antibody (1A11) [H00001820-M01] - Analysis of monoclonal antibody to ARID3A on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DRIL1 Antibody (1A11) [H00001820-M01] - Analysis of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
