14068-1-AP

antibody from Invitrogen Antibodies

Targeting: ARID3A BRIGHT, DRIL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14068-1-AP

Provider

Invitrogen Antibodies

Product name

ARID3A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: FLDDLFSFM QKRGTPVNRI PIMAKQVLDL FMLYVLVTEK GGLVEVINKK LWREITKGLN LPTSITSAAF TLRTQYMEYL YPYECEKRGL SNPNELQAAI DSNRREGRRQ SFGGSLFAYS PGGAHGMLSS PKLPVSSLGL AASTNGSSIT PAPKIKKEED SAIPITVPGR LPVSLAGHPV VAAQAAAVQA AAAQAAVAAQ AAALEQLREK LESAEPPEKK MALVADEQQR LMQRALQQNF LAMAAQLPMS IRINSQASES RQDSAVNLTG TNGSNSISMS VEINGIMYTG VLFAQPPAPT PTSAPNKGGG GGGSSSSNAG GRGGNTGTSG GQAGPAGLST PSTSTSNNSL P (244-593 aa encoded by BC060828)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARID3A antibody (Product # 14068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ARID3A antibody 14068-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 14068-1-AP (ARID3A antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 14068-1-AP (ARID3A antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ARID3A (IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3200ug.