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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB403-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-3 or recombinant rat IL-3 is observed. Does not neutralize the biological activity of rhIL-3.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P01586- Isotype
- IgG
- Antibody clone number
- MP28F8
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The spleen is the site where mast cells are induced in the development of food allergy.
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
Toyoshima S, Wakamatsu E, Ishida Y, Obata Y, Kurashima Y, Kiyono H, Abe R
International immunology 2017 Jan 1;29(1):31-45
International immunology 2017 Jan 1;29(1):31-45
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
Tomaki M, Zhao LL, Sjöstrand M, Lindén A, Ichinose M, Lötvall J
Pulmonary pharmacology & therapeutics 2002;15(2):161-8
Pulmonary pharmacology & therapeutics 2002;15(2):161-8
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-3 and Neutralization by Mouse IL-3 Antibody. Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates proliferation in the NFS60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-3 Monoclonal Antibody (Catalog # MAB403). The ND50 is typically 0.05-0.15 µg/mL.
