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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- NB100-93290 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-93290, RRID:AB_1236687
- Product name
- Rabbit Polyclonal ASH1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Mutations in ASH1L confer susceptibility to Tourette syndrome.
Liu S, Tian M, He F, Li J, Xie H, Liu W, Zhang Y, Zhang R, Yi M, Che F, Ma X, Zheng Y, Deng H, Wang G, Chen L, Sun X, Xu Y, Wang J, Zang Y, Han M, Wang X, Guan H, Ge Y, Wu C, Wang H, Liang H, Li H, Ran N, Yang Z, Huang H, Wei Y, Zheng X, Sun X, Feng X, Zheng L, Zhu T, Luo W, Chen Q, Yan Y, Huang Z, Jing Z, Guo Y, Zhang X, Schaaf CP, Xing J, Wang C, Yu F, Guan JS
Molecular psychiatry 2020 Feb;25(2):476-490
Molecular psychiatry 2020 Feb;25(2):476-490
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASH1 Antibody [NB100-93290] - Detection of Human ASH1 on HeLa whole cell lysate using NB100-93290. ASH1 was also immunoprecipitated by rabbit anti-ASH1 antibody NB100-93289.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASH1 Antibody [NB100-93290] - Detection of Human and Mouse ASH1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-ASH1 antibody NB100-93290 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASH1 Antibody [NB100-93290] - Whole cell lysate (50 ug) from mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiASH1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASH1 Antibody [NB100-93290] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASH1 Antibody [NB100-93290] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
