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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- IC615P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal GM-CSF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human GM-CSF in ELISAs. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) GM-CSF, rrGM-CSF, rhM-CSF, rhG-CSF, rhIL-5-R alpha , or rhIL-3 R alpha .
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis.
Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis.
Uchida K, Nakata K, Trapnell BC, Terakawa T, Hamano E, Mikami A, Matsushita I, Seymour JF, Oh-Eda M, Ishige I, Eishi Y, Kitamura T, Yamada Y, Hanaoka K, Keicho N
Blood 2004 Feb 1;103(3):1089-98
Blood 2004 Feb 1;103(3):1089-98
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of GM-CSF in Human Dendritic Cells by Flow Cytometry. Human dendritic cells treated with LPS, Recombinant Human TNF-alpha (Catalog # 210-TA), and Recombinant Human TGF-beta 1 (Catalog # 240-B) were stained with Mouse Anti-Human GM-CSF PE-conjugated Monoclonal Antibody (Catalog # IC615P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
