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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [8]
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- Product number
- NBP1-85788 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85788, RRID:AB_11007017
- Product name
- Rabbit Polyclonal MEF2D Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat MEF2D antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MEF2D Antibody [NBP1-85788] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MEF2D Antibody [NBP1-85788] - Analysis in human cell line THP-1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MEF2D Antibody [NBP1-85788] - Lysates from NIH3T3 and SVG-A cells assessed by ELISA, loading the indicated mass of total protein per well. Antibody was diluted 1:250, and used in conjunction with an HRP-conjugated secondary antibody. Signal was detected by luminescence. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Staining of human skeletal muscle shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Staining of human placenta.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Analysis in human skeletal muscle and liver tissues. Corresponding MEF2D RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Staining of human kidney, liver, placenta and skeletal muscle using Anti-MEF2D antibody NBP1-85788 (A) shows similar protein distribution across tissues to independent antibody NBP1-85795 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Staining of human liver shows no positivity in hepatocytes as expected.
