H00051466-M01

antibody from Abnova Corporation

Targeting: EVL RNB6

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00051466-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00051466-M01, RRID:AB_489911

Product name

EVL monoclonal antibody (M01), clone 5G1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant EVL.

Antigen sequence

PGTRAASQPPNSSEAGRKPWERSNSVEKPVSSILS
RTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALD
AFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELS
GISTT


Isotype

IgG

Antibody clone number

5G1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EVL expression in transfected 293T cell line by EVL monoclonal antibody (M01), clone 5G1.Lane 1: EVL transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EVL is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to EVL on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol