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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-53468 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC10A6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KRRLKNKHGK KNSGCTEVCH TRKSTSSRET NAFLEVNEEG AITPGPPGPM DCHRALEPVG HITSCE
- Concentration
- 0.1 mg/mL
Submitted references Cholesterol sulfate alleviates ulcerative colitis by promoting cholesterol biosynthesis in colonic epithelial cells.
Xu D, Ma R, Ju Y, Song X, Niu B, Hong W, Wang R, Yang Q, Zhao Z, Zhang Y, Zheng Y, Bai Q, Lv M, Sun N, Li X
Nature communications 2022 Jul 30;13(1):4428
Nature communications 2022 Jul 30;13(1):4428
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of SLC10A6 in human esophogial tissue shows strong positivity in basal layer of squamous epithelial cells. Samples were probed using a SLC10A6 Polyclonal Antibody (Product # PA5-53468).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SLC10A6 in human esophagus and skeletal muscle tissues using SLC10A6 Polyclonal Antibody (Product # PA5-53468). Corresponding SLC10A6 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SLC10A6 in human skeletal muscle using SLC10A6 Polyclonal Antibody (Product # PA5-53468) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 CS promotes cholesterol biosynthesis by binding to NPC2 and activating SREBP2. a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis for the up-regulated differentially expressed genes in CS-treated HT-29 cells compared with control HT-29 cells (treated with DMSO). (Fold change >=1.5, P < 0.05). b Heatmaps for the steroid biosynthesis genes in the upregulated genes group in CS-treated HT-29 cells compared with control HT-29 cells (treated with DMSO). c RT-qPCR analysis of HMGCS1 , DHCR7 , FDFT1 and CYP51A1 mRNA levels in HT-29 cells, LOVO cells, SW480 cells, HCT116 cells, SW1116 cells, and NCM460 cells treated with CS (50 muM) or DMSO for 24 h ( n = 2 independent culture wells, two-tailed Student's t- test). d The cholesterol concentration of HT-29 cells treated with CS (50 muM) for 6, 12, 24, and 48 h measured by the Amplex(r) Red Cholesterol Assay Kit ( n = 4 biologically independent samples, one-way ANOVA with Sidak's multiple comparison test). e Filipin III staining in HT-29 and SULT2B1 -KO HT-29 cells in the presence or absence of CS (50 muM) ( n = 4 fields/group, one-way ANOVA with Sidak's multiple comparison test). f Cell viability of HT-29 and SULT2B1 -KO cells treated with 25 muM or 50 muM CS ( n = 4, 3, 3 biologically independent samples/group, respectively, one-way ANOVA with Sidak's multiple comparison test). g Transcriptional profiles of SLC family members in colonic tissues from Sult2b1 f/f and Sult2b1 IEC mice challenged with 2.5% DSS ( n =
