- MA1-23244 - Invitrogen Antibodies COPS5 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of JAB1 using 30 µg of 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a JAB1 monoclonal antibody (Product # MA1-23244) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of JAB1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a JAB1 Monoclonal Antibody (2A10) (Product # MA1-23244) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using JAB1 Monoclonal Antibody (2A10) (Product # MA1-23244). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. JAB1 Monoclonal Antibody (2A10) (Product # MA1-23244) diluted at 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using JAB1 Monoclonal Antibody (2A10) (Product # MA1-23244). Sample (50 µg of whole cell lysate). Lane A: Rat liver. 10% SDS PAGE. JAB1 Monoclonal Antibody (2A10) (Product # MA1-23244) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using anti-JAB1 Monoclonal Antibody (Product # MA1-23244) and a ~37 kDa band corresponding to JAB1 was observed across the cell lines and tissues tested. An additional un-characterized band (*) was observed at ~90 kDa. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), A549 (Lane 2), HEK-293 (Lane 3), HeLa (Lane 4), MCF-7 (Lane 5), HT-29 (Lane 6), SW480 (Lane 7), HCT 116 (Lane 8), Mouse Lung (Lane 9), Rat Lung (Lane 10) and Mouse Spleen (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of JAB1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with JAB1 Monoclonal Antibody (Product # MA1-23244) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis using JAB1 Monoclonal Antibody (2A10) (Product # MA1-23244).

MA1-23244