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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [4]
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- Product number
- GTX70203 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70203, RRID:AB_371978
- Product name
- Jab1 antibody [2A10.8]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Drosophila
- Host
- Mouse
Submitted references Cul3 neddylation is crucial for gradual lipid droplet formation during adipogenesis.
COP1 enhances ubiquitin-mediated degradation of p27Kip1 to promote cancer cell growth.
The β-catenin E3 ubiquitin ligase SIAH-1 is regulated by CSN5/JAB1 in CRC cells.
The D-dopachrome tautomerase (DDT) gene product is a cytokine and functional homolog of macrophage migration inhibitory factor (MIF).
Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development.
Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5.
CSN5/Jab1 mutations affect axis formation in the Drosophila oocyte by activating a meiotic checkpoint.
The TRC8 hereditary kidney cancer gene suppresses growth and functions with VHL in a common pathway.
Expression of phosphorylated p27(Kip1) protein and Jun activation domain-binding protein 1 in human pituitary tumors.
Dubiel D, Bintig W, Kähne T, Dubiel W, Naumann M
Biochimica et biophysica acta. Molecular cell research 2017 Aug;1864(8):1405-1412
Biochimica et biophysica acta. Molecular cell research 2017 Aug;1864(8):1405-1412
COP1 enhances ubiquitin-mediated degradation of p27Kip1 to promote cancer cell growth.
Choi HH, Phan L, Chou PC, Su CH, Yeung SC, Chen JS, Lee MH
Oncotarget 2015 Aug 14;6(23):19721-34
Oncotarget 2015 Aug 14;6(23):19721-34
The β-catenin E3 ubiquitin ligase SIAH-1 is regulated by CSN5/JAB1 in CRC cells.
Jumpertz S, Hennes T, Asare Y, Vervoorts J, Bernhagen J, Schütz AK
Cellular signalling 2014 Sep;26(9):2051-9
Cellular signalling 2014 Sep;26(9):2051-9
The D-dopachrome tautomerase (DDT) gene product is a cytokine and functional homolog of macrophage migration inhibitory factor (MIF).
Merk M, Zierow S, Leng L, Das R, Du X, Schulte W, Fan J, Lue H, Chen Y, Xiong H, Chagnon F, Bernhagen J, Lolis E, Mor G, Lesur O, Bucala R
Proceedings of the National Academy of Sciences of the United States of America 2011 Aug 23;108(34):E577-85
Proceedings of the National Academy of Sciences of the United States of America 2011 Aug 23;108(34):E577-85
Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development.
McCall CM, Miliani de Marval PL, Chastain PD 2nd, Jackson SC, He YJ, Kotake Y, Cook JG, Xiong Y
Molecular and cellular biology 2008 Sep;28(18):5621-33
Molecular and cellular biology 2008 Sep;28(18):5621-33
Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5.
Yun J, Tomida A, Andoh T, Tsuruo T
The Journal of biological chemistry 2004 Jul 23;279(30):31296-303
The Journal of biological chemistry 2004 Jul 23;279(30):31296-303
CSN5/Jab1 mutations affect axis formation in the Drosophila oocyte by activating a meiotic checkpoint.
Doronkin S, Djagaeva I, Beckendorf SK
Development (Cambridge, England) 2002 Nov;129(21):5053-64
Development (Cambridge, England) 2002 Nov;129(21):5053-64
The TRC8 hereditary kidney cancer gene suppresses growth and functions with VHL in a common pathway.
Gemmill RM, Bemis LT, Lee JP, Sozen MA, Baron A, Zeng C, Erickson PF, Hooper JE, Drabkin HA
Oncogene 2002 May 16;21(22):3507-16
Oncogene 2002 May 16;21(22):3507-16
Expression of phosphorylated p27(Kip1) protein and Jun activation domain-binding protein 1 in human pituitary tumors.
Korbonits M, Chahal HS, Kaltsas G, Jordan S, Urmanova Y, Khalimova Z, Harris PE, Farrell WE, Claret FX, Grossman AB
The Journal of clinical endocrinology and metabolism 2002 Jun;87(6):2635-43
The Journal of clinical endocrinology and metabolism 2002 Jun;87(6):2635-43
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Rat liver 10% SDS PAGE GTX70203 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE GTX70203 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX70203 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Jab1 antibody detects Jab1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with Jab1 antibody (GTX70203) diluted at a dilution of 1:500.
