LS-C407703

antibody from LSBio

Targeting: COPS5 CSN5, JAB1, MOV-34, SGN5

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C407703

Provider

LSBio

Product name

COPS5 / JAB1 Antibody (aa8-334) LS-C407703

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ICC analysis of JAB1 using anti-JAB1 antibody. JAB1 was detected in immunocytochemical section of A431 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-JAB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ICC analysis of JAB1 using anti-JAB1 antibody. JAB1 was detected in immunocytochemical section of A431 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-JAB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

JAB1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.