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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX117561 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX117561, RRID:AB_11165271
- Product name
- HP1 gamma antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Histone H1 acetylation at lysine 85 regulates chromatin condensation and genome stability upon DNA damage.
Li Y, Li Z, Dong L, Tang M, Zhang P, Zhang C, Cao Z, Zhu Q, Chen Y, Wang H, Wang T, Lv D, Wang L, Zhao Y, Yang Y, Wang H, Zhang H, Roeder RG, Zhu WG
Nucleic acids research 2018 Sep 6;46(15):7716-7730
Nucleic acids research 2018 Sep 6;46(15):7716-7730
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE GTX117561 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody (GTX117561) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody (GTX117561) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HP1 gamma(GTX117561) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HP1 gamma antibody immunoprecipitates HP1 gamma protein in IP experiments.IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extractB. Control with 2 £gg of preimmune rabbit IgGC. Immunoprecipitation of HP1 gamma protein by 2 £gg of HP1 gamma antibody (GTX117561)15% SDS-PAGE The immunoprecipitated HP1 gamma protein was detected by HP1 gamma antibody (GTX117561) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using HP1 gamma(GTX117561) antibody at 1:250 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HP1 gamma antibody detects HP1 gamma protein at nucleus in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HP1 gamma antibody (GTX117561) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HP1 gamma antibody detects HP1 gamma protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HP1 gamma antibody (GTX117561) diluted at 1:500.
