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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- ELISA [1]
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- Product number
- H00054751-M10 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00054751-M10, RRID:AB_2105809
- Product name
- Mouse Monoclonal Migfilin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. FBLIM1 - filamin binding LIM protein 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Kindlin-1 Is required for RhoGTPase-mediated lamellipodia formation in keratinocytes.
Has C, Herz C, Zimina E, Qu HY, He Y, Zhang ZG, Wen TT, Gache Y, Aumailley M, Bruckner-Tuderman L
The American journal of pathology 2009 Oct;175(4):1442-52
The American journal of pathology 2009 Oct;175(4):1442-52
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Migfilin Antibody (5E11) [H00054751-M10] - Analysis of FBLIM1 expression in transfected 293T cell line by FBLIM1 monoclonal antibody (M10), clone 5E11. Lane 1: FBLIM1 transfected lysatE (41 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Migfilin Antibody (5E11) [H00054751-M10] - Analysis of FBLIM1 expression in HepG2 (Cat # L019V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Migfilin Antibody (5E11) [H00054751-M10] - Detection limit for recombinant GST tagged FBLIM1 is approximately 0.03ng/ml as a capture antibody.
