Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- sc-10750 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-10750, RRID:AB_2073888
- Product name
- Anti-CCNT1
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Cyclin-dependent Kinases Phosphorylate the Cytomegalovirus RNA Export Protein pUL69 and Modulate Its Nuclear Localization and Activity.
Cyclin T2 is essential for mouse embryogenesis.
Role of cdk9 in the optimization of expression of the genes regulated by ICP22 of herpes simplex virus 1.
Inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinases cdk9 and cdk7 at the viral transcriptosome.
Differential chromatin looping regulates CD4 expression in immature thymocytes.
Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy.
Direct inhibition of CDK9 blocks HIV-1 replication without preventing T-cell activation in primary human peripheral blood lymphocytes.
Inhibition of Tat activity by the HEXIM1 protein.
Human cytomegalovirus infection induces specific hyperphosphorylation of the carboxyl-terminal domain of the large subunit of RNA polymerase II that is associated with changes in the abundance, activity, and localization of cdk9 and cdk7.
Functional inactivation of Cdk9 through oligomerization chain reaction
CDK9 is constitutively expressed throughout the cell cycle, and its steady-state expression is independent of SKP2.
Rechter S, Scott GM, Eickhoff J, Zielke K, Auerochs S, Müller R, Stamminger T, Rawlinson WD, Marschall M
The Journal of biological chemistry 2009 Mar 27;284(13):8605-13
The Journal of biological chemistry 2009 Mar 27;284(13):8605-13
Cyclin T2 is essential for mouse embryogenesis.
Kohoutek J, Li Q, Blazek D, Luo Z, Jiang H, Peterlin BM
Molecular and cellular biology 2009 Jun;29(12):3280-5
Molecular and cellular biology 2009 Jun;29(12):3280-5
Role of cdk9 in the optimization of expression of the genes regulated by ICP22 of herpes simplex virus 1.
Durand LO, Roizman B
Journal of virology 2008 Nov;82(21):10591-9
Journal of virology 2008 Nov;82(21):10591-9
Inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinases cdk9 and cdk7 at the viral transcriptosome.
Kapasi AJ, Spector DH
Journal of virology 2008 Jan;82(1):394-407
Journal of virology 2008 Jan;82(1):394-407
Differential chromatin looping regulates CD4 expression in immature thymocytes.
Jiang H, Peterlin BM
Molecular and cellular biology 2008 Feb;28(3):907-12
Molecular and cellular biology 2008 Feb;28(3):907-12
Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy.
Espinoza-Derout J, Wagner M, Shahmiri K, Mascareno E, Chaqour B, Siddiqui MA
Cardiovascular research 2007 Jul 1;75(1):129-38
Cardiovascular research 2007 Jul 1;75(1):129-38
Direct inhibition of CDK9 blocks HIV-1 replication without preventing T-cell activation in primary human peripheral blood lymphocytes.
Salerno D, Hasham MG, Marshall R, Garriga J, Tsygankov AY, Graña X
Gene 2007 Dec 15;405(1-2):65-78
Gene 2007 Dec 15;405(1-2):65-78
Inhibition of Tat activity by the HEXIM1 protein.
Fraldi A, Varrone F, Napolitano G, Michels AA, Majello B, Bensaude O, Lania L
Retrovirology 2005 Jul 2;2:42
Retrovirology 2005 Jul 2;2:42
Human cytomegalovirus infection induces specific hyperphosphorylation of the carboxyl-terminal domain of the large subunit of RNA polymerase II that is associated with changes in the abundance, activity, and localization of cdk9 and cdk7.
Tamrakar S, Kapasi AJ, Spector DH
Journal of virology 2005 Dec;79(24):15477-93
Journal of virology 2005 Dec;79(24):15477-93
Functional inactivation of Cdk9 through oligomerization chain reaction
Giuliana Napolitano, Alberto Mazzocco, Alessandro Fraldi, Barbara Majello, Luigi Lania
Oncogene 2003 Jul;22(31):4882-4888
Oncogene 2003 Jul;22(31):4882-4888
CDK9 is constitutively expressed throughout the cell cycle, and its steady-state expression is independent of SKP2.
Garriga J, Bhattacharya S, Calbó J, Marshall RM, Truongcao M, Haines DS, Graña X
Molecular and cellular biology 2003 Aug;23(15):5165-73
Molecular and cellular biology 2003 Aug;23(15):5165-73
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 81
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
