PA5-77892

antibody from Invitrogen Antibodies

Targeting: CCNT1 CCNT, CYCT1, HIVE1

Antibody data

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Validation data

Product number: PA5-77892 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cyclin T1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Positive Control: 293T, A431, HeLa, HepG2 Predicted Reactivity: Mouse (86%), Rat (87%), Chimpanzee (100%), Bovine (84%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.0 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CCNT1 protein structure - PA5-77892 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Cyclin T1 in whole cell lysate using 50 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with Cyclin T1 polyclonal antibody (Product # PA5-77892) using a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Cyclin T1 Polyclonal Antibody (Product # PA5-77892). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cyclin T1 Polyclonal Antibody (Product # PA5-77892) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Cyclin T1 Polyclonal Antibody (Product # PA5-77892). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cyclin T1 Polyclonal Antibody (Product # PA5-77892) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Cyclin T1 Polyclonal Antibody (Product # PA5-77892). Various whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cyclin T1 Polyclonal Antibody (Product # PA5-77892) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Cyclin T1 Polyclonal Antibody(Product # PA5-77892) and a 80kDa band corresponding to Cyclin T1 was observed across cell lines along with uncharacterized bands (*). Whole Cell Extract (1% SDS) (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), Jurkat (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5) and IMR-32 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).