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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C789033 - Provider product page
- Provider
- LSBio
- Product name
- EDAG / HEMGN Antibody (aa260-484, clone OTI2D7, Carrier-free) LS-C789033
- Antibody type
- Monoclonal
- Description
- Purified from ascites.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2D7
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HEMGN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HEMGN.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Human liver tissue using anti-HEMGN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
