NBP1-84907

antibody from Novus Biologicals

Targeting: HEMGN CT155, EDAG, NDR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-84907

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-84907, RRID:AB_11041953

Product name

Rabbit Polyclonal Hemogen Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Hemogen antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Hemogen Antibody [NBP1-84907] - Analysis in control (vector only transfected HEK293T lysate) and HEMGN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human bone marrow shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human cerebral cortex shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human kidney.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Analysis in human bone marrow and cerebral cortex tissues. Corresponding HEMGN RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human colon shows no positivity in glandular cells as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human kidney shows no positivity in cells in tubules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human bone marrow, colon, kidney and testis using Anti-HEMGN antibody (A) NBP1-84907 shows similar protein distribution across tissues to independent antibody NBP1-84909 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.