AF3619

antibody from R&D Systems

Targeting: FOXJ1 FKHL13, HFH-4, HFH4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3619

Provider

R&D Systems

Product name

Human FoxJ1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human FoxJ1 in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q92949


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Gefitinib, an EGFR Tyrosine Kinase inhibitor, Prevents Smoke-Mediated Ciliated Airway Epithelial Cell Loss and Promotes Their Recovery.

Valencia-Gattas M, Conner GE, Fregien NL
PloS one 2016;11(8):e0160216

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Hyaluronan fragments/CD44 mediate oxidative stress-induced MUC5B up-regulation in airway epithelium.

Casalino-Matsuda SM, Monzon ME, Day AJ, Forteza RM
American journal of respiratory cell and molecular biology 2009 Mar;40(3):277-85

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FoxJ1 by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxJ1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FoxJ1 by Simple Western^TM. Simple Western lane view shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxJ1 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FoxJ1 in HEK293 Human Cell Line. FoxJ1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FoxJ1 in Early Proximal Lung Progenitors. FoxJ1 was detected in immersion fixed BGO1V human embryonic stem cells differentiated to early proximal lung progenitors using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.