702603

antibody from Invitrogen Antibodies

Targeting: GRM4 GPRC1D, mGlu4, MGLUR4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

702603

Provider

Invitrogen Antibodies

Product name

mGLuR4 Recombinant Rabbit Monoclonal Antibody (6H26L4)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

6H26L4

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on HEK-293 cells stably transfected with human mGLuR4 (Lane 2) and Mock (Lane 1). Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. The blot was probed with Anti-mGLuR4 Recombinant Rabbit Monoclonal Antibody (Product # 702603, 1 µg/mL, 1:500 dilution). 110 kDa and 220 kDa bands corresponding to the monomeric and dimeric forms of mGLuR4 was observed in the transfected cell line.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, HEK-293 cells were stably transfected with human mGLuR4. After 24hr of transfection the cells were fixed and permeabilized for detection of endogenous mGLuR4 using Anti-mGLuR4 Recombinant Rabbit Monoclonal Antibody (Product # 702603, 1 µg/mL, 1:500 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Panel a) shows representative cells that were stained for detection of membrane localization of the mGLuR4 on the transfected cells. The images were captured at 40X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sections of Rat cerebellum & cortex were dewaxed, microwaved in citric acid and incubated with Anti- mGLuR4 Recombinant Rabbit Monoclonal Antibody (Product # 702603, 1 µg/mL, 1:500 dilution). Sections were then sequentially treated with biotinylated Anti-rabbit IgG and AB solution. Sections were further developed in AEC and lightly counterstained with hematoxylin. Panel a & b) shows the specific localization of mGLuR4 in neuronal stomata and dendrites in cerebellum and cortex respectively. Panel c) shows the no primary antibody control.