PAB20849

antibody from Abnova Corporation

Targeting: SPEN KIAA0929, MINT, RBM15C, SHARP

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20849

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20849, RRID:AB_10965226

Product name

SPEN polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SPEN.

Antigen sequence

NRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDP
RERTLQHGLYYASRSRSPNRFDAHDPRYEPRAREQ
FTLPSVVHRDIYRDDITREVRGRRPERNYQHSRSR
SPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRS
SSSDSI


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with SPEN polyclonal antibody (Cat # PAB20849) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with SPEN polyclonal antibody (Cat # PAB20849) shows strong nuclear and slightly weaker cytoplasmic positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)