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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [1]
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- Product number
- NB100-58799 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-58799, RRID:AB_2195563
- Product name
- Rabbit Polyclonal SPEN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references SPEN protein expression and interactions with chromatin in mouse testicular cells.
Korfanty J, Stokowy T, Chadalski M, Toma-Jonik A, Vydra N, Widłak P, Wojtaś B, Gielniewski B, Widlak W
Reproduction (Cambridge, England) 2018 Sep;156(3):195-206
Reproduction (Cambridge, England) 2018 Sep;156(3):195-206
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: SPEN Antibody [NB100-58799] - Detection of Human SHARP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SHARP antibody NB100-58799 used for IP at 3 mcg/mg lysate. SHARP was also immunoprecipitated by rabbit anti-SHARP antibody NB100-58798, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes.
