NBP1-90334

antibody from Novus Biologicals

Targeting: HSD17B11 17-BETA-HSD11, 17-BETA-HSDXI, DHRS8, PAN1B, RetSDR2, SDR16C2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-90334

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-90334, RRID:AB_11018624

Product name

Rabbit Polyclonal hydroxysteroid (17-beta) dehydrogenase 11 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human hydroxysteroid (17-beta) dehydrogenase 11 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

RNA deep sequencing as a tool for selection of cell lines for systematic subcellular localization of all human proteins.

Danielsson F, Wiking M, Mahdessian D, Skogs M, Ait Blal H, Hjelmare M, Stadler C, Uhlén M, Lundberg E
Journal of proteome research 2013 Jan 4;12(1):299-307

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Hep-G2

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Simple Western: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Simple Western lane view shows a specific band for hydroxysteroid (17-beta) dehydrogenase 11 in 0.2 mg/ml of HepG2 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Simple Western: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Electropherogram image of the corresponding Simple Western lane view. hydroxysteroid (17-beta) dehydrogenase 11 antibody was used at 1:25 dilution on HepG2 lysate(s) respectively.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Analysis in human small intestine and skeletal muscle tissues. Corresponding HSD17B11 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: hydroxysteroid (17-beta) dehydrogenase 11 Antibody [NBP1-90334] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.