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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0439 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0439, RRID:AB_982438
- Product name
- CDK7 (phospho T170) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDK7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA.
Transcription factor TFIIH components enhance the GR coactivator activity but not the cell cycle-arresting activity of the human immunodeficiency virus type-1 protein Vpr.
The cyclin H/cdk7/Mat1 kinase activity is regulated by CK2 phosphorylation of cyclin H.
Zhou M, Deng L, Kashanchi F, Brady JN, Shatkin AJ, Kumar A
Proceedings of the National Academy of Sciences of the United States of America 2003 Oct 28;100(22):12666-71
Proceedings of the National Academy of Sciences of the United States of America 2003 Oct 28;100(22):12666-71
Transcription factor TFIIH components enhance the GR coactivator activity but not the cell cycle-arresting activity of the human immunodeficiency virus type-1 protein Vpr.
Kino T, Tsukamoto M, Chrousos G
Biochemical and biophysical research communications 2002 Oct 18;298(1):17-23
Biochemical and biophysical research communications 2002 Oct 18;298(1):17-23
The cyclin H/cdk7/Mat1 kinase activity is regulated by CK2 phosphorylation of cyclin H.
Schneider E, Kartarius S, Schuster N, Montenarh M
Oncogene 2002 Aug 1;21(33):5031-7
Oncogene 2002 Aug 1;21(33):5031-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The CDK7 (phospho T170) polyclonal antibody (Cat # PAB0439) is used in Western blot to detect Phospho-CDK7-T170 in Ramos cell lysate
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDK7 (phospho T170) polyclonal antibody (Cat # PAB0439). Western blot analysis of CDK7 expression in human kidney.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDK7 (phospho T170) polyclonal antibody (Cat # PAB0439) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of CDK7 (phospho T170) polyclonal antibody (Cat # PAB0439) on nitrocellulose membrane. 50 ng of phospho-peptide or non phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5 ug per mL.
- Validation comment
- Dot Blot (Peptide)
