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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [104]
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- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX70228 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70228, RRID:AB_372854
- Product name
- Rad50 antibody [13B3]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
Submitted references NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation.
Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells.
Serotype-specific restriction of wild-type adenoviruses by the cellular Mre11-Rad50-Nbs1 complex.
4β-Hydroxywithanolide E selectively induces oxidative DNA damage for selective killing of oral cancer cells.
ATM directs DNA damage responses and proteostasis via genetically separable pathways.
Homeodomain Proteins Directly Regulate ATM Kinase Activity.
Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response.
The Rb binding domain of HPV31 E7 is required to maintain high levels of DNA repair factors in infected cells.
Interplay with the Mre11-Rad50-Nbs1 complex and phosphorylation by GSK3β implicate human B-Myb in DNA-damage signaling.
Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral latency.
MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.
DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway.
AluY-mediated germline deletion, duplication and somatic stem cell reversion in UBE2T defines a new subtype of Fanconi anemia.
Enhancer of Rudimentary Homolog Affects the Replication Stress Response through Regulation of RNA Processing.
The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
Stemness factor Sall4 is required for DNA damage response in embryonic stem cells.
Human RAD50 makes a functional DNA-binding complex.
FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis.
MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.
The use of Olaparib (AZD2281) potentiates SN-38 cytotoxicity in colon cancer cells by indirect inhibition of Rad51-mediated repair of DNA double-strand breaks.
Mutations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination.
Distinct functions of human cohesin-SA1 and cohesin-SA2 in double-strand break repair.
PI3K Inhibition Augments the Therapeutic Efficacy of a 3a-aza-Cyclopenta[α]indene Derivative in Lung Cancer Cells
PI3K Inhibition Augments the Therapeutic Efficacy of a 3a-aza-Cyclopenta[α]indene Derivative in Lung Cancer Cells.
siRNA screening identifies differences in the Fanconi anemia pathway in BALB/c-Trp53+/- with susceptibility versus C57BL/6-Trp53+/- mice with resistance to mammary tumors.
Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex.
A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia.
The bromodomain protein Brd4 insulates chromatin from DNA damage signalling.
Host DNA damage response facilitates African swine fever virus infection.
CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes.
Genotyping of fanconi anemia patients by whole exome sequencing: advantages and challenges.
Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment.
Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin.
Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly.
Human T-lymphotropic type 1 virus p30 inhibits homologous recombination and favors unfaithful DNA repair.
Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death.
CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors.
SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype.
Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.
DNA damage responses in human induced pluripotent stem cells and embryonic stem cells.
Telomerase-dependent oncolytic adenovirus sensitizes human cancer cells to ionizing radiation via inhibition of DNA repair machinery.
Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents.
Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response.
53BP1 promotes ATM activity through direct interactions with the MRN complex.
Physical interaction between the histone acetyl transferase Tip60 and the DNA double-strand breaks sensor MRN complex.
Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex.
Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation.
Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes.
Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways.
Human papillomaviruses activate the ATM DNA damage pathway for viral genome amplification upon differentiation.
Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder.
MRE11 complex links RECQ5 helicase to sites of DNA damage.
Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection.
Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase.
Distinct requirements of adenovirus E1b55K protein for degradation of cellular substrates.
Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation.
Characterization of mre11 loss following HSV-1 infection.
Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins.
Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene.
Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres.
Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors.
A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends.
RINT-1 serves as a tumor suppressor and maintains Golgi dynamics and centrosome integrity for cell survival.
The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins.
Adenoviral E1B55K oncoprotein sequesters candidate leukemia suppressor sequence-specific single-stranded DNA-binding protein 2 into aggresomes.
Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone.
PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex.
Telomere lengthening early in development.
The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication.
Inhibition of hsp90 compromises the DNA damage response to radiation.
Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks.
Microsatellite instability and mutation analysis of candidate genes in urothelial cell carcinomas of upper urinary tract.
Evaluation of RAD50 in familial breast cancer predisposition.
A new type of radiosensitive T-B-NK+ severe combined immunodeficiency caused by a LIG4 mutation.
An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification.
Rapid activation of ATR by ionizing radiation requires ATM and Mre11.
The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1.
Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas.
Adenovirus exploits the cellular aggresome response to accelerate inactivation of the MRN complex.
Analysis of ionizing radiation-induced foci of DNA damage repair proteins.
Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells.
DNA repair proteins affect the lifecycle of herpes simplex virus 1.
Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies.
Expression of ATM, p53, and the MRE11-Rad50-NBS1 complex in myoepithelial cells from benign and malignant proliferations of the breast.
Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response.
The human Rap1 protein complex and modulation of telomere length.
Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability.
Slow progression of ataxia-telangiectasia with double missense and in frame splice mutations.
Altered expression of DNA double-strand break detection and repair proteins in breast carcinomas.
NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors.
Requirement of the MRN complex for ATM activation by DNA damage.
Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells.
Regulation of RelA (p65) function by the large subunit of replication factor C.
MDC1 is a mediator of the mammalian DNA damage checkpoint.
The Mre11 complex is required for ATM activation and the G2/M checkpoint.
DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein.
Specific recruitment of human cohesin to laser-induced DNA damage.
Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex.
hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage.
Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest.
The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50[middle dot]hMRE11[middle dot]NBS1 complex DNA repair activity.
Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome.
A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage.
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
Anand R, Jasrotia A, Bundschuh D, Howard SM, Ranjha L, Stucki M, Cejka P
The EMBO journal 2019 Apr 1;38(7)
The EMBO journal 2019 Apr 1;38(7)
Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells.
Fujiwara C, Muramatsu Y, Nishii M, Tokunaka K, Tahara H, Ueno M, Yamori T, Sugimoto Y, Seimiya H
Scientific reports 2018 Oct 4;8(1):14827
Scientific reports 2018 Oct 4;8(1):14827
Serotype-specific restriction of wild-type adenoviruses by the cellular Mre11-Rad50-Nbs1 complex.
Pancholi NJ, Weitzman MD
Virology 2018 May;518:221-231
Virology 2018 May;518:221-231
4β-Hydroxywithanolide E selectively induces oxidative DNA damage for selective killing of oral cancer cells.
Tang JY, Huang HW, Wang HR, Chan YC, Haung JW, Shu CW, Wu YC, Chang HW
Environmental toxicology 2018 Mar;33(3):295-304
Environmental toxicology 2018 Mar;33(3):295-304
ATM directs DNA damage responses and proteostasis via genetically separable pathways.
Lee JH, Mand MR, Kao CH, Zhou Y, Ryu SW, Richards AL, Coon JJ, Paull TT
Science signaling 2018 Jan 9;11(512)
Science signaling 2018 Jan 9;11(512)
Homeodomain Proteins Directly Regulate ATM Kinase Activity.
Johnson TE, Lee JH, Myler LR, Zhou Y, Mosley TJ, Yang SH, Bio-Bricks for Molecular Machines FRI Stream, Uprety N, Kim J, Paull TT
Cell reports 2018 Aug 7;24(6):1471-1483
Cell reports 2018 Aug 7;24(6):1471-1483
Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response.
Li Z, Li J, Kong Y, Yan S, Ahmad N, Liu X
Cancer research 2017 Jun 15;77(12):3169-3180
Cancer research 2017 Jun 15;77(12):3169-3180
The Rb binding domain of HPV31 E7 is required to maintain high levels of DNA repair factors in infected cells.
Johnson BA, Aloor HL, Moody CA
Virology 2017 Jan;500:22-34
Virology 2017 Jan;500:22-34
Interplay with the Mre11-Rad50-Nbs1 complex and phosphorylation by GSK3β implicate human B-Myb in DNA-damage signaling.
Henrich SM, Usadel C, Werwein E, Burdova K, Janscak P, Ferrari S, Hess D, Klempnauer KH
Scientific reports 2017 Jan 27;7:41663
Scientific reports 2017 Jan 27;7:41663
Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral latency.
Mariggiò G, Koch S, Zhang G, Weidner-Glunde M, Rückert J, Kati S, Santag S, Schulz TF
PLoS pathogens 2017 Apr;13(4):e1006335
PLoS pathogens 2017 Apr;13(4):e1006335
MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.
Staples CJ, Barone G, Myers KN, Ganesh A, Gibbs-Seymour I, Patil AA, Beveridge RD, Daye C, Beniston R, Maslen S, Ahel I, Skehel JM, Collis SJ
Cell reports 2016 Sep 6;16(10):2565-2575
Cell reports 2016 Sep 6;16(10):2565-2575
DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway.
Tammaro M, Liao S, Beeharry N, Yan H
Nucleic acids research 2016 Jan 8;44(1):221-31
Nucleic acids research 2016 Jan 8;44(1):221-31
AluY-mediated germline deletion, duplication and somatic stem cell reversion in UBE2T defines a new subtype of Fanconi anemia.
Virts EL, Jankowska A, Mackay C, Glaas MF, Wiek C, Kelich SL, Lottmann N, Kennedy FM, Marchal C, Lehnert E, Scharf RE, Dufour C, Lanciotti M, Farruggia P, Santoro A, Savasan S, Scheckenbach K, Schipper J, Wagenmann M, Lewis T, Leffak M, Farlow JL, Foroud TM, Honisch E, Niederacher D, Chakraborty SC, Vance GH, Pruss D, Timms KM, Lanchbury JS, Alpi AF, Hanenberg H
Human molecular genetics 2015 Sep 15;24(18):5093-108
Human molecular genetics 2015 Sep 15;24(18):5093-108
Enhancer of Rudimentary Homolog Affects the Replication Stress Response through Regulation of RNA Processing.
Kavanaugh G, Zhao R, Guo Y, Mohni KN, Glick G, Lacy ME, Hutson MS, Ascano M, Cortez D
Molecular and cellular biology 2015 Sep 1;35(17):2979-90
Molecular and cellular biology 2015 Sep 1;35(17):2979-90
The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
Harten SK, Oey H, Bourke LM, Bharti V, Isbel L, Daxinger L, Faou P, Robertson N, Matthews JM, Whitelaw E
BMC biology 2015 Mar 26;13:21
BMC biology 2015 Mar 26;13:21
Stemness factor Sall4 is required for DNA damage response in embryonic stem cells.
Xiong J, Todorova D, Su NY, Kim J, Lee PJ, Shen Z, Briggs SP, Xu Y
The Journal of cell biology 2015 Mar 2;208(5):513-20
The Journal of cell biology 2015 Mar 2;208(5):513-20
Human RAD50 makes a functional DNA-binding complex.
Kinoshita E, van Rossum-Fikkert S, Sanchez H, Kertokalio A, Wyman C
Biochimie 2015 Jun;113:47-53
Biochimie 2015 Jun;113:47-53
FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis.
Huang YL, Chou WC, Hsiung CN, Hu LY, Chu HW, Shen CY
Human molecular genetics 2015 Jun 15;24(12):3506-17
Human molecular genetics 2015 Jun 15;24(12):3506-17
MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.
Lee KY, Im JS, Shibata E, Park J, Handa N, Kowalczykowski SC, Dutta A
Nature communications 2015 Jul 28;6:7744
Nature communications 2015 Jul 28;6:7744
The use of Olaparib (AZD2281) potentiates SN-38 cytotoxicity in colon cancer cells by indirect inhibition of Rad51-mediated repair of DNA double-strand breaks.
Tahara M, Inoue T, Sato F, Miyakura Y, Horie H, Yasuda Y, Fujii H, Kotake K, Sugano K
Molecular cancer therapeutics 2014 May;13(5):1170-80
Molecular cancer therapeutics 2014 May;13(5):1170-80
Mutations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination.
Ohara M, Funyu Y, Ebara S, Sakamoto Y, Seki R, Iijima K, Ohishi A, Kobayashi J, Komatsu K, Tachibana A, Tauchi H
Journal of radiation research 2014 Jul;55(4):690-8
Journal of radiation research 2014 Jul;55(4):690-8
Distinct functions of human cohesin-SA1 and cohesin-SA2 in double-strand break repair.
Kong X, Ball AR Jr, Pham HX, Zeng W, Chen HY, Schmiesing JA, Kim JS, Berns M, Yokomori K
Molecular and cellular biology 2014 Feb;34(4):685-98
Molecular and cellular biology 2014 Feb;34(4):685-98
PI3K Inhibition Augments the Therapeutic Efficacy of a 3a-aza-Cyclopenta[α]indene Derivative in Lung Cancer Cells
Sanjiv K, Chen C, Kakadiya R, Tala S, Suman S, Wu M, Chen Y, Su T, Lee T
Translational Oncology 2014 April;7(2):256-266
Translational Oncology 2014 April;7(2):256-266
PI3K Inhibition Augments the Therapeutic Efficacy of a 3a-aza-Cyclopenta[α]indene Derivative in Lung Cancer Cells.
Sanjiv K, Chen CW, Kakadiya R, Tala S, Suman S, Wu MH, Chen YH, Su TL, Lee TC
Translational oncology 2014 Apr;7(2):256-266.e5
Translational oncology 2014 Apr;7(2):256-266.e5
siRNA screening identifies differences in the Fanconi anemia pathway in BALB/c-Trp53+/- with susceptibility versus C57BL/6-Trp53+/- mice with resistance to mammary tumors.
Böhringer M, Obermeier K, Griner N, Waldraff D, Dickinson E, Eirich K, Schindler D, Hagen M, Jerry DJ, Wiesmüller L
Oncogene 2013 Nov 28;32(48):5458-70
Oncogene 2013 Nov 28;32(48):5458-70
Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex.
Lee JH, Mand MR, Deshpande RA, Kinoshita E, Yang SH, Wyman C, Paull TT
The Journal of biological chemistry 2013 May 3;288(18):12840-51
The Journal of biological chemistry 2013 May 3;288(18):12840-51
A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia.
Zhu J, Zhou L, Wu G, Konig H, Lin X, Li G, Qiu XL, Chen CF, Hu CM, Goldblatt E, Bhatia R, Chamberlin AR, Chen PL, Lee WH
EMBO molecular medicine 2013 Mar;5(3):353-65
EMBO molecular medicine 2013 Mar;5(3):353-65
The bromodomain protein Brd4 insulates chromatin from DNA damage signalling.
Floyd SR, Pacold ME, Huang Q, Clarke SM, Lam FC, Cannell IG, Bryson BD, Rameseder J, Lee MJ, Blake EJ, Fydrych A, Ho R, Greenberger BA, Chen GC, Maffa A, Del Rosario AM, Root DE, Carpenter AE, Hahn WC, Sabatini DM, Chen CC, White FM, Bradner JE, Yaffe MB
Nature 2013 Jun 13;498(7453):246-50
Nature 2013 Jun 13;498(7453):246-50
Host DNA damage response facilitates African swine fever virus infection.
Simões M, Martins C, Ferreira F
Veterinary microbiology 2013 Jul 26;165(1-2):140-7
Veterinary microbiology 2013 Jul 26;165(1-2):140-7
CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes.
Ogiwara H, Kohno T
PloS one 2012;7(12):e52810
PloS one 2012;7(12):e52810
Genotyping of fanconi anemia patients by whole exome sequencing: advantages and challenges.
Knies K, Schuster B, Ameziane N, Rooimans M, Bettecken T, de Winter J, Schindler D
PloS one 2012;7(12):e52648
PloS one 2012;7(12):e52648
Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment.
Oplustilova L, Wolanin K, Mistrik M, Korinkova G, Simkova D, Bouchal J, Lenobel R, Bartkova J, Lau A, O'Connor MJ, Lukas J, Bartek J
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3837-50
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3837-50
Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin.
Masuda Y, Noguchi K, Segawa H, Tanaka N, Katayama K, Mitsuhashi J, Sugimoto Y
Biochemical and biophysical research communications 2011 Nov 18;415(2):305-12
Biochemical and biophysical research communications 2011 Nov 18;415(2):305-12
Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly.
Matsumoto Y, Miyamoto T, Sakamoto H, Izumi H, Nakazawa Y, Ogi T, Tahara H, Oku S, Hiramoto A, Shiiki T, Fujisawa Y, Ohashi H, Sakemi Y, Matsuura S
DNA repair 2011 Mar 7;10(3):314-21
DNA repair 2011 Mar 7;10(3):314-21
Human T-lymphotropic type 1 virus p30 inhibits homologous recombination and favors unfaithful DNA repair.
Baydoun HH, Pancewicz J, Nicot C
Blood 2011 Jun 2;117(22):5897-906
Blood 2011 Jun 2;117(22):5897-906
Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death.
Luo Y, Chen AY, Qiu J
Journal of virology 2011 Jan;85(1):133-45
Journal of virology 2011 Jan;85(1):133-45
CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors.
Anderson VE, Walton MI, Eve PD, Boxall KJ, Antoni L, Caldwell JJ, Aherne W, Pearl LH, Oliver AW, Collins I, Garrett MD
Cancer research 2011 Jan 15;71(2):463-72
Cancer research 2011 Jan 15;71(2):463-72
SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype.
Stoepker C, Hain K, Schuster B, Hilhorst-Hofstee Y, Rooimans MA, Steltenpool J, Oostra AB, Eirich K, Korthof ET, Nieuwint AW, Jaspers NG, Bettecken T, Joenje H, Schindler D, Rouse J, de Winter JP
Nature genetics 2011 Feb;43(2):138-41
Nature genetics 2011 Feb;43(2):138-41
Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.
Cheng Q, Barboule N, Frit P, Gomez D, Bombarde O, Couderc B, Ren GS, Salles B, Calsou P
Nucleic acids research 2011 Dec;39(22):9605-19
Nucleic acids research 2011 Dec;39(22):9605-19
DNA damage responses in human induced pluripotent stem cells and embryonic stem cells.
Momcilovic O, Knobloch L, Fornsaglio J, Varum S, Easley C, Schatten G
PloS one 2010 Oct 15;5(10):e13410
PloS one 2010 Oct 15;5(10):e13410
Telomerase-dependent oncolytic adenovirus sensitizes human cancer cells to ionizing radiation via inhibition of DNA repair machinery.
Kuroda S, Fujiwara T, Shirakawa Y, Yamasaki Y, Yano S, Uno F, Tazawa H, Hashimoto Y, Watanabe Y, Noma K, Urata Y, Kagawa S, Fujiwara T
Cancer research 2010 Nov 15;70(22):9339-48
Cancer research 2010 Nov 15;70(22):9339-48
Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents.
Palagyi A, Neveling K, Plinninger U, Ziesch A, Targosz BS, Denk GU, Ochs S, Rizzani A, Meier D, Thasler WE, Hanenberg H, De Toni EN, Bassermann F, Schäfer C, Göke B, Schindler D, Gallmeier E
Molecular cancer 2010 May 28;9:127
Molecular cancer 2010 May 28;9:127
Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response.
Cha H, Lowe JM, Li H, Lee JS, Belova GI, Bulavin DV, Fornace AJ Jr
Cancer research 2010 May 15;70(10):4112-22
Cancer research 2010 May 15;70(10):4112-22
53BP1 promotes ATM activity through direct interactions with the MRN complex.
Lee JH, Goodarzi AA, Jeggo PA, Paull TT
The EMBO journal 2010 Feb 3;29(3):574-85
The EMBO journal 2010 Feb 3;29(3):574-85
Physical interaction between the histone acetyl transferase Tip60 and the DNA double-strand breaks sensor MRN complex.
Chailleux C, Tyteca S, Papin C, Boudsocq F, Puget N, Courilleau C, Grigoriev M, Canitrot Y, Trouche D
The Biochemical journal 2010 Feb 24;426(3):365-71
The Biochemical journal 2010 Feb 24;426(3):365-71
Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex.
Balasubramanian N, Bai P, Buchek G, Korza G, Weller SK
Journal of virology 2010 Dec;84(24):12504-14
Journal of virology 2010 Dec;84(24):12504-14
Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation.
Gomez-Godinez V, Wu T, Sherman AJ, Lee CS, Liaw LH, Zhongsheng Y, Yokomori K, Berns MW
Nucleic acids research 2010 Dec;38(22):e202
Nucleic acids research 2010 Dec;38(22):e202
Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes.
Machida K, McNamara G, Cheng KT, Huang J, Wang CH, Comai L, Ou JH, Lai MM
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):6985-98
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):6985-98
Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways.
Collaco RF, Bevington JM, Bhrigu V, Kalman-Maltese V, Trempe JP
Virology 2009 Sep 15;392(1):24-33
Virology 2009 Sep 15;392(1):24-33
Human papillomaviruses activate the ATM DNA damage pathway for viral genome amplification upon differentiation.
Moody CA, Laimins LA
PLoS pathogens 2009 Oct;5(10):e1000605
PLoS pathogens 2009 Oct;5(10):e1000605
Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder.
Waltes R, Kalb R, Gatei M, Kijas AW, Stumm M, Sobeck A, Wieland B, Varon R, Lerenthal Y, Lavin MF, Schindler D, Dörk T
American journal of human genetics 2009 May;84(5):605-16
American journal of human genetics 2009 May;84(5):605-16
MRE11 complex links RECQ5 helicase to sites of DNA damage.
Zheng L, Kanagaraj R, Mihaljevic B, Schwendener S, Sartori AA, Gerrits B, Shevelev I, Janscak P
Nucleic acids research 2009 May;37(8):2645-57
Nucleic acids research 2009 May;37(8):2645-57
Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection.
Carson CT, Orazio NI, Lee DV, Suh J, Bekker-Jensen S, Araujo FD, Lakdawala SS, Lilley CE, Bartek J, Lukas J, Weitzman MD
The EMBO journal 2009 Mar 18;28(6):652-62
The EMBO journal 2009 Mar 18;28(6):652-62
Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase.
Schwartz RA, Carson CT, Schuberth C, Weitzman MD
Journal of virology 2009 Jun;83(12):6269-78
Journal of virology 2009 Jun;83(12):6269-78
Distinct requirements of adenovirus E1b55K protein for degradation of cellular substrates.
Schwartz RA, Lakdawala SS, Eshleman HD, Russell MR, Carson CT, Weitzman MD
Journal of virology 2008 Sep;82(18):9043-55
Journal of virology 2008 Sep;82(18):9043-55
Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation.
Lakdawala SS, Schwartz RA, Ferenchak K, Carson CT, McSharry BP, Wilkinson GW, Weitzman MD
Journal of virology 2008 Sep;82(17):8362-72
Journal of virology 2008 Sep;82(17):8362-72
Characterization of mre11 loss following HSV-1 infection.
Gregory DA, Bachenheimer SL
Virology 2008 Mar 30;373(1):124-36
Virology 2008 Mar 30;373(1):124-36
Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins.
Cervelli T, Palacios JA, Zentilin L, Mano M, Schwartz RA, Weitzman MD, Giacca M
Journal of cell science 2008 Feb 1;121(Pt 3):349-57
Journal of cell science 2008 Feb 1;121(Pt 3):349-57
Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene.
Bartkova J, Tommiska J, Oplustilova L, Aaltonen K, Tamminen A, Heikkinen T, Mistrik M, Aittomäki K, Blomqvist C, Heikkilä P, Lukas J, Nevanlinna H, Bartek J
Molecular oncology 2008 Dec;2(4):296-316
Molecular oncology 2008 Dec;2(4):296-316
Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres.
Zhong ZH, Jiang WQ, Cesare AJ, Neumann AA, Wadhwa R, Reddel RR
The Journal of biological chemistry 2007 Oct 5;282(40):29314-22
The Journal of biological chemistry 2007 Oct 5;282(40):29314-22
Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors.
You KT, Li LS, Kim NG, Kang HJ, Koh KH, Chwae YJ, Kim KM, Kim YK, Park SM, Jang SK, Kim H
PLoS biology 2007 May;5(5):e109
PLoS biology 2007 May;5(5):e109
A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends.
Bae NS, Baumann P
Molecular cell 2007 May 11;26(3):323-34
Molecular cell 2007 May 11;26(3):323-34
RINT-1 serves as a tumor suppressor and maintains Golgi dynamics and centrosome integrity for cell survival.
Lin X, Liu CC, Gao Q, Zhang X, Wu G, Lee WH
Molecular and cellular biology 2007 Jul;27(13):4905-16
Molecular and cellular biology 2007 Jul;27(13):4905-16
The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins.
Potts PR, Yu H
Nature structural & molecular biology 2007 Jul;14(7):581-90
Nature structural & molecular biology 2007 Jul;14(7):581-90
Adenoviral E1B55K oncoprotein sequesters candidate leukemia suppressor sequence-specific single-stranded DNA-binding protein 2 into aggresomes.
Fleisig HB, Orazio NI, Liang H, Tyler AF, Adams HP, Weitzman MD, Nagarajan L
Oncogene 2007 Jul 19;26(33):4797-805
Oncogene 2007 Jul 19;26(33):4797-805
Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone.
Bentle MS, Reinicke KE, Dong Y, Bey EA, Boothman DA
Cancer research 2007 Jul 15;67(14):6936-45
Cancer research 2007 Jul 15;67(14):6936-45
PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex.
Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler GR, Copeland TD, Kalkum M, Ge K
The Journal of biological chemistry 2007 Jul 13;282(28):20395-406
The Journal of biological chemistry 2007 Jul 13;282(28):20395-406
Telomere lengthening early in development.
Liu L, Bailey SM, Okuka M, Muñoz P, Li C, Zhou L, Wu C, Czerwiec E, Sandler L, Seyfang A, Blasco MA, Keefe DL
Nature cell biology 2007 Dec;9(12):1436-41
Nature cell biology 2007 Dec;9(12):1436-41
The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication.
Schwartz RA, Palacios JA, Cassell GD, Adam S, Giacca M, Weitzman MD
Journal of virology 2007 Dec;81(23):12936-45
Journal of virology 2007 Dec;81(23):12936-45
Inhibition of hsp90 compromises the DNA damage response to radiation.
Dote H, Burgan WE, Camphausen K, Tofilon PJ
Cancer research 2006 Sep 15;66(18):9211-20
Cancer research 2006 Sep 15;66(18):9211-20
Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks.
Takemura H, Rao VA, Sordet O, Furuta T, Miao ZH, Meng L, Zhang H, Pommier Y
The Journal of biological chemistry 2006 Oct 13;281(41):30814-23
The Journal of biological chemistry 2006 Oct 13;281(41):30814-23
Microsatellite instability and mutation analysis of candidate genes in urothelial cell carcinomas of upper urinary tract.
Mongiat-Artus P, Miquel C, Van der Aa M, Buhard O, Hamelin R, Soliman H, Bangma C, Janin A, Teillac P, van der Kwast T, Praz F
Oncogene 2006 Mar 30;25(14):2113-8
Oncogene 2006 Mar 30;25(14):2113-8
Evaluation of RAD50 in familial breast cancer predisposition.
Tommiska J, Seal S, Renwick A, Barfoot R, Baskcomb L, Jayatilake H, Bartkova J, Tallila J, Kaare M, Tamminen A, Heikkilä P, Evans DG, Eccles D, Aittomäki K, Blomqvist C, Bartek J, Stratton MR, Nevanlinna H, Rahman N
International journal of cancer 2006 Jun 1;118(11):2911-6
International journal of cancer 2006 Jun 1;118(11):2911-6
A new type of radiosensitive T-B-NK+ severe combined immunodeficiency caused by a LIG4 mutation.
van der Burg M, van Veelen LR, Verkaik NS, Wiegant WW, Hartwig NG, Barendregt BH, Brugmans L, Raams A, Jaspers NG, Zdzienicka MZ, van Dongen JJ, van Gent DC
The Journal of clinical investigation 2006 Jan;116(1):137-45
The Journal of clinical investigation 2006 Jan;116(1):137-45
An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification.
Oishi H, Kitagawa H, Wada O, Takezawa S, Tora L, Kouzu-Fujita M, Takada I, Yano T, Yanagisawa J, Kato S
The Journal of biological chemistry 2006 Jan 6;281(1):20-6
The Journal of biological chemistry 2006 Jan 6;281(1):20-6
Rapid activation of ATR by ionizing radiation requires ATM and Mre11.
Myers JS, Cortez D
The Journal of biological chemistry 2006 Apr 7;281(14):9346-50
The Journal of biological chemistry 2006 Apr 7;281(14):9346-50
The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1.
Kong LJ, Meloni AR, Nevins JR
Molecular cell 2006 Apr 7;22(1):63-71
Molecular cell 2006 Apr 7;22(1):63-71
Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas.
Koh KH, Kang HJ, Li LS, Kim NG, You KT, Yang E, Kim H, Kim HJ, Yun CO, Kim KS, Kim H
Laboratory investigation; a journal of technical methods and pathology 2005 Sep;85(9):1130-8
Laboratory investigation; a journal of technical methods and pathology 2005 Sep;85(9):1130-8
Adenovirus exploits the cellular aggresome response to accelerate inactivation of the MRN complex.
Liu Y, Shevchenko A, Shevchenko A, Berk AJ
Journal of virology 2005 Nov;79(22):14004-16
Journal of virology 2005 Nov;79(22):14004-16
Analysis of ionizing radiation-induced foci of DNA damage repair proteins.
van Veelen LR, Cervelli T, van de Rakt MW, Theil AF, Essers J, Kanaar R
Mutation research 2005 Jul 1;574(1-2):22-33
Mutation research 2005 Jul 1;574(1-2):22-33
Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells.
Kim JS, Krasieva TB, Kurumizaka H, Chen DJ, Taylor AM, Yokomori K
The Journal of cell biology 2005 Aug 1;170(3):341-7
The Journal of cell biology 2005 Aug 1;170(3):341-7
DNA repair proteins affect the lifecycle of herpes simplex virus 1.
Lilley CE, Carson CT, Muotri AR, Gage FH, Weitzman MD
Proceedings of the National Academy of Sciences of the United States of America 2005 Apr 19;102(16):5844-9
Proceedings of the National Academy of Sciences of the United States of America 2005 Apr 19;102(16):5844-9
Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies.
Fasching CL, Bower K, Reddel RR
Cancer research 2005 Apr 1;65(7):2722-9
Cancer research 2005 Apr 1;65(7):2722-9
Expression of ATM, p53, and the MRE11-Rad50-NBS1 complex in myoepithelial cells from benign and malignant proliferations of the breast.
Angèle S, Jones C, Reis Filho JS, Fulford LG, Treilleux I, Lakhani SR, Hall J
Journal of clinical pathology 2004 Nov;57(11):1179-84
Journal of clinical pathology 2004 Nov;57(11):1179-84
Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response.
Wilkinson DE, Weller SK
Journal of virology 2004 May;78(9):4783-96
Journal of virology 2004 May;78(9):4783-96
The human Rap1 protein complex and modulation of telomere length.
O'Connor MS, Safari A, Liu D, Qin J, Songyang Z
The Journal of biological chemistry 2004 Jul 2;279(27):28585-91
The Journal of biological chemistry 2004 Jul 2;279(27):28585-91
Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability.
Giannini G, Rinaldi C, Ristori E, Ambrosini MI, Cerignoli F, Viel A, Bidoli E, Berni S, D'Amati G, Scambia G, Frati L, Screpanti I, Gulino A
Oncogene 2004 Apr 8;23(15):2640-7
Oncogene 2004 Apr 8;23(15):2640-7
Slow progression of ataxia-telangiectasia with double missense and in frame splice mutations.
Dörk T, Bendix-Waltes R, Wegner RD, Stumm M
American journal of medical genetics. Part A 2004 Apr 30;126A(3):272-7
American journal of medical genetics. Part A 2004 Apr 30;126A(3):272-7
Altered expression of DNA double-strand break detection and repair proteins in breast carcinomas.
Angèle S, Treilleux I, Brémond A, Tanière P, Hall J
Histopathology 2003 Oct;43(4):347-53
Histopathology 2003 Oct;43(4):347-53
NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors.
Xu X, Stern DF
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2003 Oct;17(13):1842-8
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2003 Oct;17(13):1842-8
Requirement of the MRN complex for ATM activation by DNA damage.
Uziel T, Lerenthal Y, Moyal L, Andegeko Y, Mittelman L, Shiloh Y
The EMBO journal 2003 Oct 15;22(20):5612-21
The EMBO journal 2003 Oct 15;22(20):5612-21
Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells.
Hassane DC, Lee RB, Pickett CL
Infection and immunity 2003 Jan;71(1):541-5
Infection and immunity 2003 Jan;71(1):541-5
Regulation of RelA (p65) function by the large subunit of replication factor C.
Anderson LA, Perkins ND
Molecular and cellular biology 2003 Jan;23(2):721-32
Molecular and cellular biology 2003 Jan;23(2):721-32
MDC1 is a mediator of the mammalian DNA damage checkpoint.
Stewart GS, Wang B, Bignell CR, Taylor AM, Elledge SJ
Nature 2003 Feb 27;421(6926):961-6
Nature 2003 Feb 27;421(6926):961-6
The Mre11 complex is required for ATM activation and the G2/M checkpoint.
Carson CT, Schwartz RA, Stracker TH, Lilley CE, Lee DV, Weitzman MD
The EMBO journal 2003 Dec 15;22(24):6610-20
The EMBO journal 2003 Dec 15;22(24):6610-20
DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein.
Pichierri P, Averbeck D, Rosselli F
Human molecular genetics 2002 Oct 1;11(21):2531-46
Human molecular genetics 2002 Oct 1;11(21):2531-46
Specific recruitment of human cohesin to laser-induced DNA damage.
Kim JS, Krasieva TB, LaMorte V, Taylor AM, Yokomori K
The Journal of biological chemistry 2002 Nov 22;277(47):45149-53
The Journal of biological chemistry 2002 Nov 22;277(47):45149-53
Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex.
Stracker TH, Carson CT, Weitzman MD
Nature 2002 Jul 18;418(6895):348-52
Nature 2002 Jul 18;418(6895):348-52
hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage.
Richie CT, Peterson C, Lu T, Hittelman WN, Carpenter PB, Legerski RJ
Molecular and cellular biology 2002 Dec;22(24):8635-47
Molecular and cellular biology 2002 Dec;22(24):8635-47
Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest.
Franchitto A, Pichierri P
The Journal of cell biology 2002 Apr 1;157(1):19-30
The Journal of cell biology 2002 Apr 1;157(1):19-30
The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50[middle dot]hMRE11[middle dot]NBS1 complex DNA repair activity.
Tauchi H, Kobayashi J, Morishima K, Matsuura S, Nakamura A, Shiraishi T, Ito E, Masnada D, Delia D, Komatsu K
The Journal of biological chemistry 2001 Jan 5;276(1):12-5
The Journal of biological chemistry 2001 Jan 5;276(1):12-5
Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome.
Harfst E, Cooper S, Neubauer S, Distel L, Grawunder U
Molecular immunology 2000 Oct;37(15):915-29
Molecular immunology 2000 Oct;37(15):915-29
A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage.
Paull TT, Rogakou EP, Yamazaki V, Kirchgessner CU, Gellert M, Bonner WM
Current biology : CB 2000 Jul 27-Aug 10;10(15):886-95
Current biology : CB 2000 Jul 27-Aug 10;10(15):886-95
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J
Genes & development 2000 Apr 15;14(8):927-39
Genes & development 2000 Apr 15;14(8):927-39
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rad50 antibody [13B3] detects Rad50 protein by western blot analysis.A. 30 ?g HeLa whole cell lysate/extract5% SDS-PAGERad50 antibody [13B3] (GTX70228) dilution: 1:1000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX70228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rad50 protein stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
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- Main image
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- Rad50 antibody [13B3] detects Rad50 protein at nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
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- Main image
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- Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
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- GeneTex (provider)
- Main image
- Experimental details
- Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC-3 xenograft. Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
