TA321721

antibody from OriGene

Targeting: RAD50 hRad50, RAD50-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321721

Provider

OriGene

Product name

Anti-RAD50 Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-RAD50 Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

RAD50

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1.3mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 154 kDa . Positive control: 293T, Hela, K562, NIH/3T3 and RAW264.7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lane3: K562 cell lysate Lane4: NIH/3T3 cell lysate Lane5: RAW264.7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human gastric cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using RAD50 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC