NB100-56745

antibody from Novus Biologicals

Targeting: NR4A1 GFRP1, HMR, N10, NAK-1, NGFIB, NUR77, TR3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-56745

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-56745, RRID:AB_2153757

Product name

Rabbit Polyclonal NGFI-B alpha/Nur77/NR4A1 Antibody

Antibody type

Polyclonal

Description

Protein G purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Nr4a1 suppresses cocaine-induced behavior via epigenetic regulation of homeostatic target genes.

Carpenter MD, Hu Q, Bond AM, Lombroso SI, Czarnecki KS, Lim CJ, Song H, Wimmer ME, Pierce RC, Heller EA
Nature communications 2020 Jan 24;11(1):504

---

Transcriptional coactivator with PDZ-binding motif suppresses the expression of steroidogenic enzymes by nuclear receptor 4 A1 in Leydig cells.

Shin JH, Lee G, Jeong MG, Kim HK, Won HY, Choi Y, Lee JH, Nam M, Choi CS, Hwang GS, Hwang ES
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Apr;34(4):5332-5347

---

Bis-Indole-Derived Nuclear Receptor 4A1 (NR4A1, Nur77) Ligands as Inhibitors of Endometriosis.

Mohankumar K, Li X, Sung N, Cho YJ, Han SJ, Safe S
Endocrinology 2020 Apr 1;161(4)

---

The orphan nuclear receptor NUR77 promotes trophoblast invasion at early pregnancy through paracrine placental growth factor.

Li XC, Yin XJ, Hong W, Liu J, Jin F, Wang BY, Wang YM, Tian FJ
Journal of molecular medicine (Berlin, Germany) 2019 Sep;97(9):1359-1373

---

Differential expression of a stress-regulated gene Nr4a2 characterizes early- and late-born hippocampal granule cells.

Imura T, Kobayashi Y, Suzutani K, Ichikawa-Tomikawa N, Chiba H
Hippocampus 2019 Jun;29(6):539-549

---

Nur77 promotes cigarette smoke‑induced autophagic cell death by increasing the dissociation of Bcl2 from Beclin-1.

Qin H, Gao F, Wang Y, Huang B, Peng L, Mo B, Wang C
International journal of molecular medicine 2019 Jul;44(1):25-36

---

The nuclear orphan receptor NR4A1 regulates β1-integrin expression in pancreatic and colon cancer cells and can be targeted by NR4A1 antagonists.

Hedrick E, Lee SO, Safe S
Molecular carcinogenesis 2017 Sep;56(9):2066-2075

---

The autism-related gene SNRPN regulates cortical and spine development via controlling nuclear receptor Nr4a1.

Li H, Zhao P, Xu Q, Shan S, Hu C, Qiu Z, Xu X
Scientific reports 2016 Jul 19;6:29878

---

Structure-dependent activation of NR4A2 (Nurr1) by 1,1-bis(3'-indolyl)-1-(aromatic)methane analogs in pancreatic cancer cells.

Li X, Lee SO, Safe S
Biochemical pharmacology 2012 May 15;83(10):1445-55

---

Activation of nuclear TR3 (NR4A1) by a diindolylmethane analog induces apoptosis and proapoptotic genes in pancreatic cancer cells and tumors.

Yoon K, Lee SO, Cho SD, Kim K, Khan S, Safe S
Carcinogenesis 2011 Jun;32(6):836-42

---

Inactivation of the orphan nuclear receptor TR3/Nur77 inhibits pancreatic cancer cell and tumor growth.

Lee SO, Abdelrahim M, Yoon K, Chintharlapalli S, Papineni S, Kim K, Wang H, Safe S
Cancer research 2010 Sep 1;70(17):6824-36

---

Activation of nerve growth factor-induced B alpha by methylene-substituted diindolylmethanes in bladder cancer cells induces apoptosis and inhibits tumor growth.

Cho SD, Lee SO, Chintharlapalli S, Abdelrahim M, Khan S, Yoon K, Kamat AM, Safe S
Molecular pharmacology 2010 Mar;77(3):396-404

---

Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines.

Levin VA, Panchabhai SC, Shen L, Kornblau SM, Qiu Y, Baggerly KA
Journal of proteome research 2010 Jan;9(1):179-91

---

Paclitaxel directly binds to Bcl-2 and functionally mimics activity of Nur77.

Ferlini C, Cicchillitti L, Raspaglio G, Bartollino S, Cimitan S, Bertucci C, Mozzetti S, Gallo D, Persico M, Fattorusso C, Campiani G, Scambia G
Cancer research 2009 Sep 1;69(17):6906-14

---

p21 expression is induced by activation of nuclear nerve growth factor-induced Balpha (Nur77) in pancreatic cancer cells.

Lee SO, Chintharlapalli S, Liu S, Papineni S, Cho SD, Yoon K, Safe S
Molecular cancer research : MCR 2009 Jul;7(7):1169-78

---

Resistance of Foxp3+ regulatory T cells to Nur77-induced apoptosis promotes allograft survival.

Tao R, Hancock WW
PloS one 2008 May 28;3(5):e2321

---

1,1-Bis(3'-indolyl)-1-(p-chlorophenyl)methane activates the orphan nuclear receptor Nurr1 and inhibits bladder cancer growth.

Inamoto T, Papineni S, Chintharlapalli S, Cho SD, Safe S, Kamat AM
Molecular cancer therapeutics 2008 Dec;7(12):3825-33

---

Activation of Nur77 by selected 1,1-Bis(3'-indolyl)-1-(p-substituted phenyl)methanes induces apoptosis through nuclear pathways.

Chintharlapalli S, Burghardt R, Papineni S, Ramaiah S, Yoon K, Safe S
The Journal of biological chemistry 2005 Jul 1;280(26):24903-14

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution