MA5-42642

antibody from Invitrogen Antibodies

Targeting: CDK9 C-2k, CDC2L4, PITALRE, TAK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42642

Provider

Invitrogen Antibodies

Product name

CDK9 Recombinant Rabbit Monoclonal Antibody (1C4E3)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive test controls include: HeLa, 293T, MCF7, Mouse brain, Rat testis, Rat brain. The target is usually found in the following locations: Cytoplasm, Nucleus, PML body.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

1C4E3

Vial size

100 µL

Concentration

0.23 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CDK9 in HeLa cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CDK9 in various cell lines using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3 min.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CDK9 in HeLa cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CDK9 in HeLa cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CDK9 in human esophageal using a Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CDK9 in human esophageal using a Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CDK9 in human esophageal using a Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:100.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of CDK9 in extracts of DLD-1 cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642)The ChIP sequencing results indicate the enrichment pattern of CDK9 in selected genomic region and representative gene loci (e.g. RAC1, GAPDH and ACTB), as shown in figure.