PA5-112092

antibody from Invitrogen Antibodies

Targeting: B3GNT3 B3GN-T3, B3GNT-3, beta3Gn-T3, HP10328, TMEM3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112092

Provider

Invitrogen Antibodies

Product name

B3GNT3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of B3GNT3 using B3GNT3 Polyclonal Antibody (Product # PA5-112092) at a dilution of 1:500. Lane A: HeLa whole cell lysate, Lane B: Jurkat whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 60 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of B3GNT3 was performed on (Lane A) 0.5 mg Jurkat whole cell lysate using 4 µL of B3GNT3 Polyclonal Antibody (Product # PA5-112092) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Goat Anti-Rabbit IgG (H+L)/HRP was used as a secondary antibody at a dilution of 1:10,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 64 kDa.