PA5-90615

antibody from Invitrogen Antibodies

Targeting: HNRNPD AUF1, HNRPD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-90615

Provider

Invitrogen Antibodies

Product name

hnRNP D Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MSEEQFGGDG AAAAATAAVG GSAGEQEGAM VAATQGAAAA AGSGAGTGGG TASGGTEGGS AESEGAKIDA SKNEEDEGHS NSSPRHSEAA TAQREEWKMF IGGLSWDTTK KDLKDYFSKF GEVVDCTLKL DPITGRSRGF GFVLFKESES VDKVMDQKEH KLNGKVIDPK RAKAMKTKEP VKKIFVGGLS PDTPEEKIRE YFGGFGEVES IELPMDNKTN KRRGFCFITF KEEEPVKKIM EKKYHNVGLS KCEIKVAMSK EQYQQQQQWG SRGGFAGRAR GRGGDQQSGY GKVSRRGGHQ NSYKPY; Positive Samples: HeLa, Jurkat, HepG2, Mouse brain, Rat brain; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.88 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of hnRNP D in extracts of various cell lines using hnRNP D Polyclonal Antibody (Product # PA5-90615) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of hnRNP D was performed in C6 cells using hnRNP D Polyclonal Antibody (Product # PA5-90615) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of hnRNP D was performed in U-2 OS cells using hnRNP D Polyclonal Antibody (Product # PA5-90615).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of hnRNP D was performed in C6 cells using hnRNP D Polyclonal Antibody (Product # PA5-90615).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of hnRNP D was performed in U-2 OS cells using hnRNP D Polyclonal Antibody (Product # PA5-90615).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of hnRNP D in paraffin-embedded rat liver using hnRNP D Polyclonal Antibody (Product # PA5-90615) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of hnRNP D in paraffin-embedded human lung cancer using hnRNP D Polyclonal Antibody (Product # PA5-90615) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of hnRNP D in paraffin-embedded mouse brain using hnRNP D Polyclonal Antibody (Product # PA5-90615) at a dilution of 1:200.