M09982

antibody from Boster Biological Technology

Targeting: HNRNPD AUF1, HNRPD

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M09982

Provider

Boster Biological Technology

Product name

Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband™ (monoclonal, 2B12)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for hnRNP D/AUF1/HNRNPD detection. Tested with WB, IHC-P, ICC/IF, IF, FCM in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2B12

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of hnRNP D/AUF1/HNRNPD using anti-hnRNP D/AUF1/HNRNPD antibody (M09982). hnRNP D/AUF1/HNRNPD was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-hnRNP D/AUF1/HNRNPD Antibody (M09982) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Additional image