12085-2-AP

antibody from Invitrogen Antibodies

Targeting: HMG20A FLJ10739, HMGX1, HMGXB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12085-2-AP

Provider

Invitrogen Antibodies

Product name

HMG20A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MENLMTSST LPPLFADEDG SKESNDLATT GLNHPEVPYS SGATSSTNNP EFVEDLSQGQ LLQSESSNAA EGNEQRHEDE QRSKRGGWSK GRKRKKPLRD SNAPKSPLTG YVRFMNERRE QLRAKRPEVP FPEITRMLGN EWSKLPPEEK QRYLDEADRD KERYMKELEQ YQKTEAYKVF SRKTQDRQKG KSHRQDAARQ ATHDHEKETE VKERSVFDIP IFTEEFLNHS KAREAELRQL RKSNMEFEER NAALQKHVES MRTAVEKLEV DVIQERSRNT VLQQHLETLR QVLTSSFASM PLPGSGETPT VDTIDSYMNR LHSIILANPQ DNENFIATVR EVVNRLDR (1-347 aa encoded by BC021959)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP ( HMG20A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 12085-2-AP (HMG20A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-HMG20A (IP:12085-2-AP, 4ug; Detection:12085-2-AP 1:500) with Jurkat cells lysate 3200ug.