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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF654 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine IL-4 in ELISAs and Western blots. In direct ELISAs, this antibody shows less than 10% cross-reactivity with recombinant mouse IL-4 and recombinant rat IL-4.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q04745- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets.
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
Elahi S, Thompson DR, Van Kessel J, Babiuk LA, Gerdts V
Infection and immunity 2017 Apr;85(4)
Infection and immunity 2017 Apr;85(4)
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Nadworny PL, Wang J, Tredget EE, Burrell RE
Journal of inflammation (London, England) 2010 Feb 19;7:13
Journal of inflammation (London, England) 2010 Feb 19;7:13
Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
Journal of virology 2006 Jan;80(1):372-82
Journal of virology 2006 Jan;80(1):372-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-4 in Porcine PBMCs. IL-4 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Porcine IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Porcine IL-4 Antibody. Recombinant Porcine IL-4 (Catalog # 654-P4) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-4 (2 ng/mL) is neutralized (green line) by increasing concen-trations of Porcine IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654). The ND50 is typically 1-5 µg/mL.
