Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 500704 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#500704, RRID:AB_315116
- Product name
- PE anti-human IL-4
- Antibody type
- Monoclonal
- Antigen
- Yeast-expressed, recombinant human IL-4
- Description
- IL-4 is a pleiotropic cytokine that is produced by activated T cells mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells. The 8D4-8 antibody can neutralize the bioactivity of natural or recombinant IL-4.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 100 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.
Gong G, Shao L, Wang Y, Chen CY, Huang D, Yao S, Zhan X, Sicard H, Wang R, Chen ZW
Blood 2009 Jan 22;113(4):837-45
Blood 2009 Jan 22;113(4):837-45
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then surface stained with CD4 PE/Cy5 and intracellular stained with 8D4-8 PE
- Conjugate
- Yellow dye