PA5-121019

antibody from Invitrogen Antibodies

Targeting: KIF3A FLA10, KLP-20

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-121019

Provider

Invitrogen Antibodies

Product name

KIF3A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: MCF-7, SW480, 293T, SKOV3, HeLa, Mouse brain, Mouse ovary, Rat liver, Rat brain. The target is usually found in the following locations: Cell projection, Cytoplasm, cilium, cytoskeleton. Immunogen sequence: RKALETKLDM EEEERNKARA ELEKREKDLL KAQQEHQSLL EKLSALEKKV IVGGVDLLAK AEEQEKLLEE SNMELEERRK RAEQLRRELE EKEQERLDIE EKYTSLQEEA QGKTKKLKKV WTMLMAAKSE MADLQQEHQR EIEGLLENIR QLSRELRLQM LIIDNFIPRD YQEMIENYVH WNEDIGEWQL KCVAYTGNNM RKQTPVPDKK EKDPFEVDLS HVYLAYTEES LRQSLMKLER PRTSKGKARP KTGRRKRSAK PETVIDSLLQ

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.38 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KIF3A in MCF-7 cells using a KIF3A Polyclonal antibody (Product # PA5-121019) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KIF3A in MCF-7 cells using a KIF3A Polyclonal antibody (Product # PA5-121019) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of KIF3A in various cell lines using a KIF3A Polyclonal antibody (Product # PA5-121019) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of KIF3A in MCF7 cells using a KIF3A Polyclonal antibody (Product # PA5-121019). Blue: DAPI for nuclear staining.