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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB7568 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7568, RRID:AB_1576325
- Product name
- ILF2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of ILF2.
- Antigen sequence
C-EKAYEKPPEKKEG- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A protein interaction network for pluripotency of embryonic stem cells.
Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH
Nature 2006 Nov 16;444(7117):364-8
Nature 2006 Nov 16;444(7117):364-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ILF2 polyclonal antibody (Cat # PAB7568) (0.3 ug/mL) staining of human tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ILF2 polyclonal antibody (Cat # PAB7568) (2.5 ug/mL) staining of parental and stable knock-down cell line (fixed by 4% formaldehyse, permealized by Triton X100). Primary incubation was 1.5 hour. Detected by fluorescence.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ILF2 polyclonal antibody (Cat # PAB7568, 2.5 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
