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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [9]
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- Product number
- 24572-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NF45 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MRGDRGRGR GGRFGSRGGP GGGFRPFVPH IPFDFYLCEM AFPRVKPAPD ETSFSEALLK RNQDLAPNSA EQASILSLVT KINNVIDNLI VAPGTFEVQI EEVRQVGSYK KGTMTTGHNV ADLVVILKIL PTLEAVAALG NKVVESLRAQ DPSEVLTMLT NETGFEISSS DATVKILITT VPPNLRKLDP ELHLDIKVLQ SALAAIRHAR WFEENASQST VKVLIRLLKD LRIRFPGFEP LTPWILDLLG HYAVMNNPTR QPLALNVAYR RCLQILAAGL FLPGSVGITD PCESGNFRVH TVMTLEQQDM VCYTAQTLVR ILSHGGFRKI LGQEGDASYL ASEISTWDGV IVTPSEKAYE KPPEKKEGEE EEENTEEPPQ GEEEESMETQ E (1-390 aa encoded by BC000382)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 24572-1-AP ( NF45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 24572-1-AP (NF45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 24572-1-AP (NF45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon slide using 24572-1-AP ( NF45 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 24572-1-AP ( NF45 Antibody) at dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon slide using 24572-1-AP (NF45 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:400.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:400.
