sc-28317

antibody from Santa Cruz Biotechnology

Targeting: MCM4 CDC21, CDC54, hCdc21, MGC33310, P1-Cdc21

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-28317

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-28317, RRID:AB_627916

Product name

Anti-MCM4

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.

Lubelsky Y, Sasaki T, Kuipers MA, Lucas I, Le Beau MM, Carignon S, Debatisse M, Prinz JA, Dennis JH, Gilbert DM
Nucleic acids research 2011 Apr;39(8):3141-55

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Ki-67 expression is superior to mitotic count and novel proliferation markers PHH3, MCM4 and mitosin as a prognostic factor in thick cutaneous melanoma.

Ladstein RG, Bachmann IM, Straume O, Akslen LA
BMC cancer 2010 Apr 14;10:140

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 97

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.