NBP1-33105
antibody from Novus Biologicals
Targeting: MCM4
CDC21, CDC54, hCdc21, MGC33310, P1-Cdc21
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-33105 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33105, RRID:AB_10004031
- Product name
- Rabbit Polyclonal MCM4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM4 Antibody [NBP1-33105] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM4 Antibody [NBP1-33105] - MCM4 antibody detects MCM4 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug NCI-H929 whole cell lysate/extract. 7.5 % SDS-PAGE. MCM4 antibody dilution: 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM4 Antibody [NBP1-33105] - (30 ug of whole cell lysate) MOLT4 7. 5% SDS PAGE, antibody diluted at 1:5000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-33105] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: MCM4 Antibody [NBP1-33105] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-MCM3 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.