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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A302-304A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRD7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD7 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, HeLa, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD7 antibody (Product # A302-304A; lot A302-304A-2) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD7 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BRD7 antibody A302-304A used for WB at 0.4 µg/ml Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD7 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRD7 (Product # A302-304A; lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD7 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRD7 (Cat. No. A302-304A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD7 antibody (Product # A302-304A; lot A302-304A-2) used for IP at 6 µg per reaction. BRD7 was also immunoprecipitated by a previous lot of this antibody (lot A302-304A-1). For blotting immunoprecipitated BRD7 (Product # A302-304A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
