AF6854

antibody from R&D Systems

Targeting: FOXP4 FLJ40908

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6854

Provider

R&D Systems

Product name

Human FoxP4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human FoxP4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxP1, rhFoxP2, rhFoxP3, and rhCTBP1 is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q8IVH2


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FoxP4 by Western Blot. Western blot shows lysates of COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line either mock transfected, transfected with human FoxP1, transfected with human FoxP2, transfected with human FoxP3, or transfected with human FoxP4. PVDF membrane was probed with 1:1000 dilution of Sheep Anti-Human FoxP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6854) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. For additional reference, the membrane was co-probed with an Anti-Actin Antibody. A specific band was detected for FoxP4 at approximately 75 kDa and Actin at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions. Image courtesy of Dr. Alison Banham, Ms. Linden Lyne, and Dr. Paola Bignone, The University of Oxford, Oxford, UK.