Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 18018-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLAG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: TCKVCLQTF ESTGVLLEHL KSHAGKSSGG VKEKKHQCEH CDRRFYTRKD VRRHMVVHTG RKDFLCQYCA QRFGRKDHLT RHMKKSHNQE LLKVKTEPVD FLDPFTCNVS VPIKDELLPV MSLPSSELLS KPFTNTLQLN LYNTPFQSMQ SSGSAHQMIT TLPLGMTCPI DMDTVHPSHH LSFKYPFSST SYAISIPEKE QPLKGEIESY LMELQGGVPS SSQDSQASSS SKLGLDPQIG SLDDGAGDLS LSKSSISISD PLNTPALDFS QLFNFIPLNG PPYNPLSVGS LGMSYSQEEA HSSVSQLPPQ TQDLQDPANT IGLGSLHSLS AAFTSSLSTS TTLPRFHQAF Q (151-500 aa encoded by BC075047)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.24 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 18018-1-AP ( PLAG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 18018-1-AP (PLAG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 18018-1-AP (PLAG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PLAG1 (IP:18018-1-AP, 4ug; Detection:18018-1-AP 1:300) with HepG2 cells lysate 2400ug.